Thickened Waste Activated Sludge Research Articles

Optimizing the Mixing Ratios of Source-Separated Organic Waste and Thickened Waste Activated Sludge in Anaerobic Co-Digestion: A New Approach

Anaerobic co-digestion (AnCoD) presents several advantages over conventional mono-digestion. Various factors can impact the efficiency of the co-digestion process, including the mixing ratio of the feedstocks. This study primarily investigates the effects of different mixing ratios on methane production during the co-digestion of source-separated municipal organic waste (SSO) with thickened waste activated sludge (TWAS). While the C/N or COD/N ratio has generally been used for optimizing the mixing ratios of co-digested feedstocks, a new approach is introduced in this study to evaluate the effects of the lipid, protein, and carbohydrate (L:P:C) ratios on the efficiency of AnCoD with respect to methane production, kinetics, and synergism at mixing ratios of TWAS:SSO of 10:90, 30:70, 50:50, 70:30, and 10:90. AnCoD improved methane production and kinetics relative to TWAS at all mixing ratios, the highest of which was at the 10:90 ratio, corresponding to a methane yield, maximum methane production rate, and an L:P:C ratio of 353 mL CH4/g COD, 25 mL CH4/g COD/d, and 8:1:18, respectively. Improvements in methane yields and kinetics due to synergy were evident at all mixing ratios, with improvements in methane yields ranging from 11 to 23% and improvements in kinetics ranging from 18 to 58%. Improvements in methane yields and kinetics were insensitive to the feedstock composition beyond the 50:50 mixing ratio.

A mass balance approach for quantifying the role of natural decay and fate mechanisms on SARS-CoV-2 genetic marker removal during water reclamation.

The recent SARS-CoV-2 outbreak yielded substantial data regarding virus fate and prevalence at water reclamation facilities (WRFs), identifying influential factors as natural decay, adsorption, light, pH, salinity, and antagonistic microorganisms. However, no studies have quantified the impact of these factors in full scale WRFs. Utilizing a mass balance approach, we assessed the impact of natural decay and other fate mechanisms on genetic marker removal during water reclamation, through the use of sludge and wastewater genetic marker loading estimates. Results indicated negligible removal of genetic markers during P/PT (primary effluent (PE) p value: 0.267; preliminary and primary treatment(P/PT) accumulation p value: 0.904; and thickened primary sludge (TPS) p value: 0.076) indicating no contribution of natural decay and other fate mechanisms toward removal in P/PT. Comparably, adsorption and decomposition was found to be the dominant pathway for genetic marker removal (thickened waste activated sludge (TWAS) log loading 9.75 log10 GC/day); however, no estimation of log genetic marker accumulation could be carried out due to high detections in TWAS. PRACTITIONER POINTS: The mass balance approach suggested that the contribution of natural decay and other fate mechanisms to virus removal during wastewater treatment are negligible compared with adsorption and decomposition in P/PT (p value: 0.904). During (P/PT), a higher viral load remained in the (PE) (14.16 log10 GC/day) compared with TPS (13.83 log10 GC/day); however, no statistical difference was observed (p value: 0.280) indicting that adsorption/decomposition most probably did not occur. In secondary treatment (ST), viral genetic markers in TWAS were consistently detected (13.41 log10 GC/day) compared with secondary effluent (SE), indicating that longer HRT and the potential presence of extracellular polymeric substance-containing enriched biomass enabled adsorption/decomposition. Estimations of total solids and volatile solids for TPS and TWAS indicated that adsorption affinity was different between solids sampling locations (p value: <0.0001).

Optimised start-up strategy for bioelectrochemical systems operating on hydrolysed human urine

Key nutrients, such as nitrogen measured as total ammonium nitrogen (TAN), could be recycled from hydrolysed human urine back to fertiliser use. Bioelectrochemical systems (BESs) are an interesting, low-energy option for realising this. However, the high TAN concentration (>5 g/L) and pH (>9) of hydrolysed urine can inhibit microbial growth and hinder the enrichment of an electroactive biofilm at the anode. This study investigated a new strategy for bioanode inoculation by mixing real hydrolysed urine with thickened waste activated sludge (TWAS) from a municipal wastewater treatment plant at different volumetric ratios. The addition of TWAS diluted the high TAN concentration of hydrolysed urine (5.2 ± 0.3 g/L) to 2.6–5.1 g/L, while the pH of the inoculation mixtures remained > 9 and soluble chemical oxygen demand (sCOD) at 5.6–6.7 g/L. Despite the high pH, current generation started within 24 h for all reactors, and robust bioanodes tolerant of continuous feeding with undiluted hydrolysed urine were enriched within 11 days of start-up. Current output and Coulombic efficiency decreased with increasing initial hydrolysed urine fraction. The anodes inoculated with the highest sCOD-to-TAN ratio (2.1) performed the best, which suggests that high organics levels can protect microbes from inhibition even at elevated TAN concentrations.

Enhancing Single- and Two-Stage Anaerobic Digestion of Thickened Waste-Activated Sludge through FNA-Heat Pretreatment

This study aimed to investigate the effect of combined Free Nitrous Acid (FNA)-Heat (i.e., FNH) pretreatment on single- and two-stage anaerobic digestion (AD) of thickened waste-activated sludge (TWAS). Single-stage AD was conducted in batches, while two-stage AD involved acidogenic fermentation under semi-continuous flow followed by batch methanogenesis. FNH pretreatment was applied before the acidogenic stage, using 1.4 mg HNO2-N/L FNA concentration at 25 °C, 37 °C, and 60 °C for 24 h. Among the scenarios, the most promising results were observed with two-stage AD fed with FNH-pretreated TWAS at 60 °C, showing higher COD solubilization and a reduction in volatile solids. Combined FNA-Heat pretreatment in two-stage AD yielded elevated methane production (363–415 mL CH4/g VS added) compared to single-stage digestion. Methane yields from FNA-Heat pretreated single-stage ranged from 332 to 347 mL CH4/g VS added, contrasting with 212 mL CH4/g VS added for untreated TWAS. Methane generation commenced early in both untreated and pretreated samples, attributed to soluble substrate abundance.

Low-temperature thermal hydrolysis for enhancing sludge anaerobic digestion and antibiotic resistance management: Significance of digester solids retention time

Recently, there has been a growing inclination towards utilizing primary sludge (PS) fermentation prior to anaerobic digestion (AD) in water resource recovery facilities (WRRFs), where sludge liquor containing volatile fatty acids is used for biological nutrient removal. Nevertheless, using a low-temperature thermal hydrolysis process (THP) to improve AD in WRRFs adopting PS fermentation remains an area that has received limited research attention. Here, we studied the impact of THP (90 °C, 90 min) on anaerobic co-digestion of thickened waste activated sludge (TWAS) and fermented primary sludge (FPS) under varying solids retention times (SRTs) in semi-continuous mode. The study involved two THP schemes: scheme 1, where THP was done for both TWAS and FPS, and scheme 2, where THP was applied to TWAS only. The results demonstrated that reducing SRT from 20 to 15 and 10 d leads to decreased methane yield in both schemes. However, THP significantly enhances methane production, showing improvements of up to 37.9 % (scheme 1) and 31.2 % (scheme 2) under a 15-d SRT. Furthermore, while decreasing SRT increased the proliferation of antibiotic resistance genes (ARGs), thermal hydrolysis could effectively reduce most ARGs, indicating its potential to mitigate antibiotic resistance in the AD process. Overall, these results provide useful perceptions regarding the potential adoption of low-temperature THP in WRRFs with PS fermentation.

Supplemental Sewage Scum and Organic Municipal Solid Waste Addition to the Anaerobic Digestion of Thickened Waste Activated Sludge: Biomethane Potential and Microbiome Analysis

Sewage scum (SS) is collected from sedimentation tanks in wastewater treatment plants (WWTPs). Despite its huge biogas potential, there is limited information on its potential as a co-substrate and microbial ecology, especially during anaerobic co-digestion (ACo-D) of the organic fraction of municipal solid waste (OFMSW) and thickened waste activated sludge (TWAS). In this biomethane potential (BMP) study, the bioenergy yield achieved by the supplemental addition of SS and OFMSW to TWAS was investigated, along with the microbial ecology. Compared with the digestion of TWAS alone, which produced 184.6 mLCH4 gVS−1, biomethane yield was enhanced by as much as 32.4–121.6% in trinary mixtures with SS and OFMSW, mainly due to the positive synergistic effect. Furthermore, a mixture of 40%SS + 10%TWAS + 50%OFMSW produced the highest biogas yield of 407 mLCH4 gVS−1, which is proof that existing WWTPs can produce additional energy by incorporating external bioresources, thereby reducing greenhouse gas emissions. Modified Gompertz and logistic function estimates showed that methane production rate improved by as much as 60% in a trinary mixture compared with the digestion of TWAS alone. The genus Methanosaeta, capable of generating methane by the acetoclastic methanogenic pathway among all the archaeal communities, was the most prominent, followed by hydrogenotrophic methanogen Methanospirillum.

Novel free nitrous acid and ultrasonication pretreatment enhanced sludge biodegradability

ABSTRACT The main goal of this study was to investigate the novel combined Ultrasonication and Free Nitrous Acid (FNA) pretreatment on biodegradability and kinetics of thickened waste-activated sludge (TWAS). Partial factorial design with four levels of (0, 600, 1500, and 3000 KJ/Kg) for ultrasonication and 0, 0.7, 1.4, and 2.8  mg HNO2-N/L for FNA dose were examined creating 16 different combinations. Results revealed that combined pretreatment could significantly improve solubilization and solid destruction compared to solo pretreatments. The highest organic matter solubilization of 25.6% and volatile suspended solids destruction of 21.7% were observed when 2.8  mg HNO2-N/L and 1500 KJ/Kg were combined. Moreover, combining the pretreatments further enhanced biodegradability up to the highest percentage of 50.3% when pretreatment of 3000 KJ/Kg and 2.8  mg HNO2-N/L was applied. Also, the experimental data from a biochemical methane potential test was fitted well into First Order Kinetic and Modified Gompertz models, given that the coefficients of determination, R2, for models at all treatment levels were above 99%.

Comprehensive Review of Hydrothermal Pretreatment Parameters Affecting Fermentation and Anaerobic Digestion of Municipal Sludge

Municipal solid waste treatment and disposal have become one of the major concerns in waste management due to the excessive production of waste and higher levels of pollution. To address these challenges and protect the environment in sustainable ways, the hydrothermal pretreatment (HTP) technique coupled with anaerobic digestion (AD) becomes a preferred alternative technology that can be used for municipal solid waste stabilization and the production of renewable energy. However, the impact of HTP parameters such as temperature, retention time, pH, and solid content on the fermentation of TWAS is yet to be well studied and analyzed. Hence this study was conducted to review the effect of hydrothermal pretreatment of thickened waste-activated sludge (TWAS) on fermentation and anaerobic digestion processes. Many studies reported that fermentation of TWAS at pretreatment temperature ranges from 160 °C to 180 °C resulted in a 50% increase in volatile fatty acid (VFA) yields compared to no pretreatment. However, for the AD process, HTP in the range of 175 °C to 200 °C with a 30–60 min retention time was considered the optimal condition for higher biogas production, with 30% increase in biodegradability and greater than 55% increase in biogas production. Even though there is a direct relationship between increased HTP temperature and the hydrolysis of TWAS, a pretreatment temperature range beyond 200 °C alters the biogas production. The solid content (SC) of sludge plays a crucial role in HTP, where in practice up to 16% SC has been utilized for HTP. Further, a combined alkaline-HTP enhances the process performance.

Combined thermal hydrolysis pretreatment and anaerobic co-digestion of waste activated sludge and food waste

In this study, the impact of thermal hydrolysis pretreatment (THP) on the mono- and co-digestion of thickened waste activated sludge (TWAS) and food waste (FW) was carried out at temperatures of 150, 170, 190, and 210 °C and volumetric ratios of 90:10, 70:30, and 50:50 in batch anaerobic tests. THP enhanced solubilization, reduced volatile suspended solids (VSS) and the particle size of TWAS as the temperature increased showing optimal improvements at 170 °C. THP showed no significant impact on FW with only marginal improvements at 150 °C. Increasing the temperature beyond 170 °C for TWAS and 150 °C for FW deteriorated anaerobic biodegradability forming refractory compounds. Co-digestion improved methane yields and kinetics as the contribution of FW increased. Co-digestion of thermally pretreated TWAS with FW improved the methane yields by 27% and kinetics by 29% at 170 °C with no synergism. Co-digestion of thermally pretreated FW with TWAS improved methane yields by 15% and kinetics by 25% at 150 °C, with improvements up to 21% in synergy. THP of the mixed feedstocks improved methane yields by 53% and kinetics by 92% at 170 °C, with improvements becoming less pronounced with the increase in the volume of FW and temperature.

Anaerobic digestion of thickened waste activated sludge under calcium hypochlorite stress: Performance stability and microbial communities

Hypochlorite pretreatment has been proven effective in enhancing waste activated sludge (WAS) anaerobic digestion performances recently. In this study, two semi-continuous anaerobic sequencing batch reactors (ASBRs), one fed with Ca(ClO)2 pretreated thickened WAS (TWAS) and one with raw TWAS, were operated at mesophilic conditions (35 °C) for 145 days. Three loading shocks were introduced to each reactor to compare the performance stability and resilience between the digestion of Ca(ClO)2 pretreated TWAS and untreated TWAS. Microbial community shifts were quantified to reveal the microbiome responses to disturbances. The results suggested that 1% Ca(ClO)2 enhanced the digestion of TWAS by inactivating and transforming the biomass to more easily digested substrates. Co-occurrence network analysis revealed that the strongest interactions in the microbial community occurred in the steady state of TWAS anaerobic digestion.

Anaerobic co-digestion of municipal sludge with fat-oil-grease (FOG) enhances the destruction of sludge solids

The objective of this study was to investigate the benefits of co-digestion of a sludge-mix of primary sludge (PS)/thickened waste activated sludge (TWAS) with concentrated fat-oil-grease (FOG) over a wide range of FOG/sludge-mix volumetric feed ratios. The biodegradability (i.e., COD to methane conversion) of PS, TWAS, sludge-mix, and FOG was 43.0, 38.6, 41.8, and 97.7%, respectively, with a pseudo first-order rate of 0.13, 0.12, 0.13, and 0.18 d−1, respectively. Batch co-digestion of sludge-mix and FOG at COD ratios ranging from 93.2:6.8 to 27.3:72.7% resulted in methane production linearly correlated to both the total waste blend and FOG COD feed concentration. An enhanced extent of degradation of the sludge-mix COD to as much as 10.9% (increased from 42.2 to 53.1%) and an increased degradation rate by 17% (increased from 0.12 to 0.14 d−1) was observed when the feed FOG COD was 18.5% of the total waste COD feed. Overall, co-digestion of mixed municipal sludge with FOG is feasible and recommended to meet target biogas/methane levels at municipal wastewater treatment facilities taking into account the trade-off between energy production and solids destruction to fit their particular needs.

Enhancement of denitrification efficiency using municipal and industrial waste fermentation liquids as external carbon sources

The addition of external carbon source for nitrogen removal from wastewater is an essential step in wastewater treatment. In this study, various external carbon sources from the fermentation of primary sludge (PS), thickened waste activated sludge (TWAS), food waste (FW), bakery processing & kitchen waste (BP + KW), fat, oil, & grease (FOG), and whey powder (WP) were successfully employed for wastewater denitrification. Methanol and acetate were also used as controls due to their common use as external carbon sources for wastewater denitrification. The denitrification performance and kinetics such as the specific denitrification rate (SDNR), denitrification potential (PDN), and the biomass yield were studied at a constant TVFA as COD/N ratio of 5 for all substrates. Complete denitrification was achieved with a NO3−-N removal efficiency of 98–99%, and no NO2− accumulation was observed at the end of the experiments for all substrates. The results revealed that the liquid fermentation filtrates exhibited higher SDNRs than methanol and acetate. This indicates the high organic matter utilization efficiency and better denitrification ability of fermentation filtrates over conventional carbon sources. WP exhibited the highest SDNR of 17.6 mg NOx − N/g VSS/h, which is approximately four times that of methanol (4.6 mg NOx − N/g VSS/h). The other carbon sources had SDNRs two to three times higher than that of methanol. However, the fermentation filtrates exhibited higher biomass yields of 0.26–0.37 mg VSS/mg COD compared to methanol of 0.21 mg VSS/mg COD, which could lead to higher sludge handling costs. Moreover, methanol exhibited higher PDN of 0.25 g N/g COD compared to all the fermentation filtrates.

Combined hydrothermal and free nitrous acid, alkali and acid pretreatment for biomethane recovery from municipal sludge.

This study focused on investigating the effect of combined chemical and hydrothermal pretreatment (HTP) on the anaerobic digestibility of thickened waste activated sludge (TWAS). Three different combined pretreatment conditions of HTP+free nitrous acid (FNA), HTP+Acid, and HTP+Alkaline were applied to TWAS. To control and compare the effect of combined pretreatments and a single pretreatment, Acid, Alkaline, FNA and HTP pretreatments were applied done prior to AD. The results of this study revealed that combined pretreatments have higher potential to improve methane production yield and rate but not in the solubilization of COD. The highest methane yield of 275mL CH4/g TCOD added was achieved for the combined pretreatment with FNA and HTP. HTP+FNA pretreatment was found to produce higher methane yields compared to the combination of other typical acid and alkaline reagents with hydrothermal pretreatment. Methane yields of 594, 527, and 544 L CH4/g VSS added, were achieved for HTP+FNA, HTP+ALK, and HTP+ACID pretreatments, respectively. The preliminary economic analysis showed that out of the combined pretreatment, only combining HTP with FNA is economically feasible.

Low-temperature thermal hydrolysis for anaerobic digestion facility in wastewater treatment plant with primary sludge fermentation

Primary sludge (PS) fermentation prior to anaerobic digestion (AD) is becoming more common in wastewater treatment plants (WWTPs) for the production and utilization of volatile fatty acids-rich sludge liquor in biological nutrient removal processes. However, the thermal hydrolysis process (THP) for enhancing AD of sludge in WWTPs with PS fermentation has rarely been investigated. This study examined low-temperature THP (50–90°C, 30–90 min) for enhancing co-digestion of fermented primary sludge (FPS) and thickened waste activated sludge (TWAS). The experiments were conducted under two schemes: scheme-1 (THP of TWAS + FPS) and scheme-2 (THP of TWAS only). Scheme-2 was more effective in solubilizing COD (up to 20.4% increase in SCOD) and various macromolecular compounds (e.g., proteins and carbohydrates). In contrast, scheme-1 was more efficient in VSS solubilization (up to 26.12%). Scheme-1 also resulted in a greater enhancement in methane production over the control (scheme-1: 56.28% at 90°C, 90 min vs. scheme-2: 43.4% at 90°C, 60 min). Thus, these results suggested that low-temperature THP of TWAS alone (scheme-2) would result in solubilization of refractory macromolecular compounds, leading to a relatively lower positive impact on methane yields than scheme-1. The preliminary economic assessment considering THP operating cost, enhancement in energy recovery, and saving in biosolids handling costs indicated that THP at 90°C (90 min) under scheme-1 could provide the highest net saving of $79.55/dry tonne solids compared to the control (AD without THP). These results will provide technical guidance in adopting THP in WWTPs with PS fermentation.

Impact of AnMBR operating conditions on anaerobic digestion of waste activated sludge.

The impact of solids retention time (SRT) and hydraulic retention time (HRT) on anaerobic digestion of thickened waste activated sludge (TWAS) in a pilot-scale anaerobic membrane bioreactor (AnMBR) was compared with that achieved in conventional anaerobic digestions (CD). The AnMBR was able to successfully digest municipal TWAS at HRTs ranging from 7 to 15days and SRTs ranging from 15 to 30days. Increasing SRT in the AnMBR resulted in a significant improvement in COD and VS removal efficiency when compared against CD operating at the same HRT. The VS and COD destructions (35%-50%) observed in the AnMBR were similar to those observed in CD operating at the same SRT but longer HRTs. Operation at elevated ratios of SRT/HRT resulted in the production of a thickened biosolid (2%-3% TS). Specific methane production values for AnMBR operating at HRT-SRT ratios of 15-30, 7-30, and 7-15 were 0.19, 0.19, and 0.14m3 CH4 /kg of COD fed, respectively, showing a 25% increase in methane production with SRT. A model based upon describing hydrolysis of biodegradable solids using first-order kinetics was able to describe VS destruction as a function of SRT. PRACTITIONER POINTS: The AnMBR process was able to successfully digest waste activated sludge at a shorter seven-day HRTs Operation at elevated ratios of SRT/HRT resulted in enhanced biogas and thickened biosolid (2%-3% TS) production requiring reduced downstream processing The AnMBR process produces a particle-free permeate that might be suitable for side stream nutrient recovery A model developed by considering hydrolysis as a limiting process can be used to determine design SRTs.

Biomethane production improvement by hydrothermal pretreatment of thickened waste activated sludge.

This study evaluated the impact of hydrothermal pretreatment on thickened waste activated sludge (TWAS) for solubilization enhancement and biomethane production improvement through the mesophilic anaerobic digestion process. In order to assess the effect of temperature, retention time and severity index (SI) of the hydrothermal pretreatment, TWAS was exposed to fifteen different pretreatment conditions within a combination of 10 different pretreatment temperature range (150-240 °C), five different retention times (5-30 min) and five different severity indexes (SI = 3, 3.5, 4, 4.5 and 5). The solubilization enhancement was observed in all hydrothermally pretreated samples with the highest solubilization efficiency of 49% in pretreatment conditions of 200 °C and 10 min retention time within the corresponding SI = 4. Biomethane production was not improved in all fifteen pretreatment conditions, pretreatment with SI beyond 4 decreased the biodegradability of TWAS. The highest biomethane production was observed in the pretreatment condition of 170 °C and 10 min with a 40% increase compared to non-pretreated TWAS.

Optimization of thermal hydrolysis process for enhancing anaerobic digestion in a wastewater treatment plant with existing primary sludge fermentation

Many wastewater treatment plants (WWTPs) adopted primary sludge fermentation to produce sludge liquor for the biological denitrification process. The fermented primary sludge (FPS) is usually co-digested with thickened waste activated sludge (TWAS) in the anaerobic digestion (AD) process. To date, there has been limited information on how the sludge thermal hydrolysis process (THP) could be retrofitted for enhancing AD in WWTPs with the existing primary sludge fermentation process. This study assessed two THP retrofitting schemes, (FPS + TWAS and TWAS alone) combining different exposure times (15, 30, and 60 min) and temperatures (140, 160, and 180 °C). The results suggested that temperature had more impact on sludge solubilization than exposure times. Notably, 180 °C was the most effective for sludge solubilization under both schemes. However, a higher degree of solubilization did not necessarily lead to higher methane yields. The THP of FPS + TWAS attained considerably higher methane yield than the pretreatment of TWAS alone.

Free nitrous acid pre-treatment enhances anaerobic digestion of waste activated sludge and rheological properties of digested sludge: A pilot-scale study

In this study, the effects of free nitrous acid (FNA) pre-treatment on the rheological properties of digested sludge were investigated at a pilot-scale, along with the improvement in volatile solids (VS) destruction and biogas production. Two pilot-scale anaerobic sludge digesters were operated for one year, one receiving thickened waste activated sludge (TWAS) without pre-treatment (control) and one receiving TWAS pre-treated for 24 h at an FNA concentration of 4.9–6.1 mgN/L (nitrite = 250 mgN/L, pH = 5.0, T = 22–30 °C). The results confirmed the enhancing effect of FNA pre-treatment on methane production (37 ± 1%), consistent with previous laboratory studies. Equally importantly, FNA pre-treatment substantially reduced the shear viscosity of TWAS by 51 ± 8% at 100 s−1 and 49 ± 7% at 250 s−1, likely due to the solubilization of the TWAS (11.1 ± 0.8%). Similarly, FNA pre-treatment also reduced these viscosity parameters of the digested sludge by 80 ± 4% and 78 ± 4%, respectively, caused by both enhanced VS destruction and disintegration of the digested sludge. The dewaterability of digested sludge, assessed by dewatered solids content, capillary suction time and specific resistance to filtration, was not improved by FNA pre-treatment. The polymer requirement for dewatering was reduced by 24 ± 0.6% due to the lower solids concentration in the digested sludge achieved with FNA pre-treatment. The changes to sludge rheological properties revealed in this study further enhances the business case for the FNA pre-treatment technology.

Effect of Hydrothermal Pretreatment on Volatile Fatty Acids Production from Thickened Waste Activated Sludge

Investigating the impact of hydrothermal pretreatment (HTP) on organic solubilization and anaerobic acidification of thickened waste activated sludge (TWAS) was the objective of this study. For this purpose, 15 different hydrothermal pretreatment scenarios were investigated. The HTP temperature, retention time, and pressure for this study extended from 150 to 240 °C, 5 to 30 min, and 69 to 488 psi respectively. Mesophilic batch acidification tests were conducted for all pretreated and raw samples. The highest solubilization percentage after HTP and acidification was observed at HTP condition of “200 °C–10 min–225 psi” and “180 °C–15 min–145 psi” accounting for 48% and 40% respectively. Moreover, The HTP condition of “190 °C–10 min–181 psi” indicated the highest volatile fatty acid (VFA) production yield of 5.38 g/L or 1076.6 mgVFAs/gVSSadded. For all hydrothermally pretreated samples, acetic acid was determined to be the most abundant VFA. There was no significant hydrogen produced during the acidification process.

Increasing capacity of an anaerobic sludge digester through FNA pre-treatment of thickened waste activated sludge

Free nitrous acid (FNA) pre-treatment has been previously demonstrated to be effective in enhancing methane production and volatile solids (VS) destruction in the anaerobic digestion of waste activated sludge for an equivalent hydraulic retention time (HRT). We hypothesise that, due to enhancement of hydrolysis kinetics, FNA pre-treatment will also allow reduction in the HRT while retaining performance. This would allow for improvement of capacity constrained digesters. Two anaerobic sludge digesters (control-experiment) were fed with the same thickened waste activated sludge (TWAS) from a full-scale plant for 6 months. With 24 h pre-treatment of TWAS at an FNA concentration of 6.1 mgN/L (NO2-N = 250 mg/L, pH = 5.0, T = 25 °C), the HRT for the experimental anaerobic digester was progressively reduced from 15 days to 12 days and then to 7.5 days. In comparison, the control reactor was operated at a constant HRT of 15 days, representing typical loading conditions. With the shortened HRTs, the experimental AD reactor achieved VS destruction at 36.9 ± 0.8% (12 days) and 36.8 ± 1.0% (7.5 days), representing 30–40% relative increase in comparison to the control reactor (at 26.5 ± 0.8% and 28.3 ± 0.7%, respectively, in the same two periods). This was supported by a similar (31–35%) increase in the methane production per unit of VS fed. The volumetric methane production rate of the experimental digester was increased by 165% at HRT of 7.5 days compared with the control digester at HRT of 15 days. The results demonstrated that FNA pre-treatment of TWAS can substantially increase the capacity of an anaerobic sludge digester, with a highly favourable economic outcome.